Nasopharyngeal brushing: a convenient and feasible sampling method for nucleic acid-based nasopharyngeal carcinoma research

Table 1 Primer and probe sequences used in qPCR for TSGs and EBV BamHI-W detection

Primer	Sequences	Tm (°C)	Amplicon length (bp)
CACNA2D3	Forward, 5′-CAGCTGCCTCTGTGAATCTGTGG-3′	63.58	288
CACNA2D3	Reverse, 5′-ATGATTTAGCATGCCAAGAGAACATC-3′	60.24	288
PRDM5	Forward, 5′-CAGGTTCTCCCTGAAGTCCT-3′	58.35	249
PRDM5	Reverse, 5′-TGAGATGGTGCCTCATGAAC-3′	57.59	249
WIF1	Forward, 5′-TGAATTCCTGTCCTTGCGCT-3′	59.96	296
WIF1	Reverse, 5′-ACTCGCAGATGCGTCTTTCA-3′	60.04	296
RIZ1	Forward, 5′-TGAATCAGAACACTACTGAGCC-3′	57.81	297
RIZ1	Reverse, 5′-GCAGCCAGTTTCCCTTCTCT-3′	59.96	297
CHFR	Forward, 5′-AACCGGAACACAGGTCTGG-3′	59.55	247
CHFR	Reverse, 5′-TGGACGGTTTGGGCATTTCT-3′	60.18	247
RASFF1A	Forward, 5′-CGCGCATTGCAAGTTCACC-3′	61.08	242
RASFF1A	Reverse, 5′-AGCCTGTGTAAGAACCGTCC-3′	59.68	242
RPS13	Forward, 5′-TCGGCTTTACCCTATCGACGCAG-3′	64.47	153
RPS13	Reverse, 5′-ACGTACTTGTGCAACACCATGTGA-3′	63.24	153
BamHI-W	Forward, 5′-CCCAACACTCCACCACACC-3′	60.53	76
BamHI-W	Reverse, 5′-TCTTAGGAGCTGTCCGAGGG-3′	60.40	76
Hybridization probe	5′-FAMCACACACTACACACACCCACCCGTCTC-TAMRA-3′

qPCR quantitative polymerase chain reaction, EBV Epstein–Barr virus, CACNA2D3 calcium channel regulatory subunit α2δ3, PRDM5 positive regulatory domain I-binding factor 1 and retinoblastoma protein-interacting zinc-finger gene domain-containing protein 5, WIF1 WNT inhibitory factor 1, RIZ1 retinoblastoma protein-interacting zinc finger gene 1, CHFR checkpoint with forkhead and ring finger domains, RASFF1A Ras association domain family 1A, RPS13 ribosomal protein S13

ISSN: 2523-3548